Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance.
Nat Biotechnol
; 22(7): 856-62, 2004 Jul.
Article
em En
| MEDLINE
| ID: mdl-15195103
ABSTRACT
Crop loss due to viral diseases is still a major problem for agriculture today. We present a strategy to achieve virus resistance based on the expression of single-chain Fv fragments (scFvs) against a conserved domain in a plant viral RNA-dependent RNA polymerase (RdRp), a key enzyme in virus replication. The selected scFvs inhibited complementary RNA synthesis of different plant virus RdRps in vitro and virus replication in planta. Moreover, the scFvs also bound to the RdRp of the distantly related hepatitis C virus. T(1) and T(2) progeny of transgenic lines of Nicotiana benthamiana expressing different scFvs either in the cytosol or in the endoplasmic reticulum showed varying degrees of resistance against four plant viruses from different genera, three of which belong to the Tombusviridae family. Virus resistance based on antibodies to RdRps adds another tool to the repertoire for combating plant viruses.
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Bases de dados:
MEDLINE
Assunto principal:
Doenças das Plantas
/
Vírus de Plantas
/
Replicação Viral
/
RNA Polimerase Dependente de RNA
/
Anticorpos Antivirais
Idioma:
En
Revista:
Nat Biotechnol
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Alemanha