Fast-gated intensified charge-coupled device camera to record time-resolved fluorescence spectra of tryptophan.
Appl Spectrosc
; 58(6): 705-10, 2004 Jun.
Article
em En
| MEDLINE
| ID: mdl-15198823
ABSTRACT
The possibilities of a 200 ps gated intensified charge-coupled device (CCD) camera to record time-resolved fluorescence were explored using the fluorescing amino acid tryptophan and its derivative Nacetyl-tryptophan amide (NATA) as model compounds. The results were compared to complementary data from time-correlated single-photon counting (TCSPC) experiments. If a spectral resolution of 1-2 nm is desired, the fast-gated intensified CCD (ICCD) camera is the method of choice. For a 10(-5) M tryptophan solution, time-resolved emission spectra and intensity decays (measured over 12 ns at 25 ps resolution) could be obtained in typically 10 minutes, giving the well-known lifetimes of 0.5 and 3 ns. In addition, a longer lifetime of 7 ns was found at the red edge of the spectrum. The very short gate time of the ICCD camera allowed us to observe a shift in the emission maximum of tryptophan even within the first nanosecond of decay of the fluorescence emission. As expected from the tryptophan rotamer model, such a shift is not observed in NATA. Using amplitudes obtained by global analysis, decay-associated spectra of these lifetimes were constructed.
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Bases de dados:
MEDLINE
Assunto principal:
Espectrometria de Fluorescência
/
Triptofano
Tipo de estudo:
Evaluation_studies
/
Prognostic_studies
Idioma:
En
Revista:
Appl Spectrosc
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Holanda