Induction of protective immunity against lethal anthrax challenge with a patch.
J Infect Dis
; 190(4): 774-82, 2004 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-15272406
ABSTRACT
BACKGROUND:
Transcutaneous immunization (TCI) is a needle-free technique that delivers antigens and adjuvants to potent epidermal immune cells. To address critical unmet needs in biodefense against anthrax, we have designed a novel vaccine delivery system using a dry adhesive patch that simplifies administration and improves tolerability of a subunit anthrax vaccine.METHODS:
Mice and rabbits were vaccinated with recombinant protective antigen of Bacillus anthracis and the heat-labile toxin of Escherichia coli. Serologic changes, levels of toxin-neutralizing antibodies (TNAs), and pulmonary and nodal responses were monitored in the mice. A lethal aerosolized B. anthracis challenge model was used in A/J mice, to demonstrate efficacy.RESULTS:
The level of systemic immunity and protection induced by TCI was comparable to that induced by intramuscular vaccination, and peak immunity could be achieved with only 2 doses. The addition of adjuvant in the patch induced superior TNA levels, compared with injected vaccination.CONCLUSIONS:
Anthrax vaccine patches stimulated robust and functional immune responses that protected against lethal challenge. Demonstration of responses in the lung suggests that a mechanism exists for protection against challenge with aerosolized anthrax spores. A formulated, pressure-sensitive, dry adhesive patch, which is stable and can be manufactured in large scale, elicited comparable immunoglobulin G and TNA responses, suggesting that an anthrax vaccine patch is feasible and should advance into clinical evaluation.
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Bases de dados:
MEDLINE
Assunto principal:
Bacillus anthracis
/
Vacinação
/
Vacinas contra Antraz
/
Proteínas de Escherichia coli
/
Antraz
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Infect Dis
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Estados Unidos