Neurogenic cells in human amniotic fluid.
Am J Obstet Gynecol
; 191(1): 309-14, 2004 Jul.
Article
em En
| MEDLINE
| ID: mdl-15295384
ABSTRACT
OBJECTIVE:
The purpose of this study was to determine whether human amniotic fluid contains cells that harbor the potential to differentiate into neurogenic cells. STUDYDESIGN:
Amniotic fluid cells (uncultivated or cultivated in standard or in neurogenic differentiation medium) were analyzed for morphologic neurogenic differentiation and for expression of cluster of differentiation 133 (marker for neuronal stem cells), nestin (neuronal progenitor cells), neurofilament (neurons), the p75 common neurotrophin receptor, the brain-derived neurotrophic factor and neurotrophin-3 and cyclic nucleotide phosphodiesterase (oligodendrocytes).RESULTS:
The appearance of neurogenic cells was not detected in uncultivated cells, was sporadic after cultivation in standard medium but strongly increased in neurogenic differentiation medium, and was accompanied by the induction of the expression of the analyzed marker genes.CONCLUSION:
For the first time, this study provides evidence that human amniotic fluid contains cells that express markers for neuronal stem and progenitor cells, which harbor the potential to differentiate into neurogenic cells.
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Bases de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Diferenciação Celular
/
Fator Neurotrófico Derivado do Encéfalo
/
Líquido Amniótico
/
Proteínas do Tecido Nervoso
Limite:
Female
/
Humans
Idioma:
En
Revista:
Am J Obstet Gynecol
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Áustria