Porcine amelogenin is expressed from the X and Y chromosomes.
J Dent Res
; 84(2): 144-8, 2005 Feb.
Article
em En
| MEDLINE
| ID: mdl-15668331
ABSTRACT
Amelogenin is the major enamel matrix component in developing teeth. In eutherian mammals, amelogenin is expressed from the X chromosome only, or from both the X and Y chromosomes. Two classes of porcine amelogenin cDNA clones have been characterized, but the chromosomal localization of the gene(s) encoding them is unknown. To determine if there are sex-based differences in the expression of porcine amelogenin, we paired PCR primers for exons 1a, 1b, 7a, and 7b, and amplified enamel organ-derived cDNA separately from porcine males and females. The results show that exons 1a/2a and 7a are always together and can be amplified from both males (XY) and females (XX). Exons 1b/2b and 7b are also always paired, but can be amplified only from females. We conclude that porcine amelogenin is expressed from separate genes on the X and Y chromosomes, and not, as previously proposed, from a single gene with two promoters.
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Bases de dados:
MEDLINE
Assunto principal:
Germe de Dente
/
Cromossomo X
/
Cromossomo Y
/
Proteínas do Esmalte Dentário
/
Órgão do Esmalte
Limite:
Animals
Idioma:
En
Revista:
J Dent Res
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Japão