Microarray analysis of genes that respond to gamma-irradiation in Arabidopsis.
J Agric Food Chem
; 53(4): 1022-30, 2005 Feb 23.
Article
em En
| MEDLINE
| ID: mdl-15713015
ABSTRACT
To reveal the signal transduction mechanism of the response to stress in the form of active oxygen species, we used a microarray system to analyze gene expression patterns 2 or 24 h after gamma-irradiation of Arabidopsis. gamma-Irradiation induces several signal transduction and metabolite genes. By analysis of cis-elements located on the promoter region of the gamma-responsive genes, we have also found several cis-elements related to various signal transduction systems. We also analyzed the pleiotropic mutant ttg1-1, which has a dramatically altered physiological response to gamma-irradiation. By comparing the gene expression patterns of wild-type (Ler) and ttg1-1 mutant plants after gamma-irradiation, we identified various TTG1-regulated gamma-response genes. Analysis of the cis-elements in the promoter region of the gamma-responsive genes also revealed that the many transcription factors interacting with TTG1 protein (WD40 protein) are related to the gamma-responsive gene expression.
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Bases de dados:
MEDLINE
Assunto principal:
Expressão Gênica
/
Genes de Plantas
/
Arabidopsis
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Raios gama
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Agric Food Chem
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Japão