Viability and adipogenic potential of human adipose tissue processed cell population obtained from pump-assisted and syringe-assisted liposuction.
J Dermatol Sci
; 37(3): 169-76, 2005 Mar.
Article
em En
| MEDLINE
| ID: mdl-15734286
ABSTRACT
BACKGROUND:
Correcting soft tissue defects by autologous fat grafting has been a routine procedure in plastic surgery. Liposuction material for autologous fat grafting is normally obtained using a hand-held syringe. The pump-assisted liposuction technique is not used because of the belief that cells in the harvested fat tissue are necrotic from the physical forces created by the vacuum pump liposuction machine.OBJECTIVE:
The metabolism and adipogenic potential of cultured mesenchymal precursor cells processed from pump and syringe lipoaspirates were studied.METHODS:
Metabolic estimates were determined with alamarBlue assay. These cells were also induced along the adipogenic lineage with adipogenic induction factors. The extent of adipogenic differentiation was measured using a digital photo counting software.RESULTS:
The cells obtained by pump-assisted liposuction are viable, have comparable metabolic activity and adipogenic potential which are comparable to cells using the syringe for aspiration.CONCLUSION:
The implications of this study are that pump-assisted liposuction might be a possible alternative to aspirating adipose tissue for reimplantation during reconstruction procedures.
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Bases de dados:
MEDLINE
Assunto principal:
Lipectomia
/
Tecido Adiposo
Limite:
Humans
Idioma:
En
Revista:
J Dermatol Sci
Assunto da revista:
DERMATOLOGIA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Singapura