[Mechanism of urotensin II-stimulated adrenomedullin secretion in human vascular endothelial cells].
Di Yi Jun Yi Da Xue Xue Bao
; 25(7): 791-3, 2005 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-16027069
ABSTRACT
OBJECTIVE:
To study the mechanism of urotensin II(U II)-stimulated adrenomedullin secretion in human vascular endothelial cells.METHODS:
In cultured human vascular endothelial cells (HEVCs), different concentrations of U II was used to stimulate the secretion of Adm, and different inhibitors were used to study the changes in the secretion after block of different signal transduction pathways. The contents of Adm in the medium were detected with radioimmunoassay.RESULTS:
U II-stimulated Adm secretion in the HEVCs in a time- and concentration-dependent manner. Adm contents of the treatment groups were comparable with that of the control group (P<0.05 ), and the secretion of Adm could be inhibited by the inhibitor of extracellular signal-regulated protein kinases (PD098059), p38 kinase inhibitor (SB202190), calmodulin inhibitor (W7) and Ca(2+) inhibitor (nicardipine)(P<0.05), but calcineurin inhibitor and protein kinase C inhibitor (H7) had no such effect (P>0.05).CONCLUSION:
Ca(2+), MAPK, CaM-PK and p38 signal transduction pathways may play major roles in U II-stimulated secretion of Adm in HVECs.
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Bases de dados:
MEDLINE
Assunto principal:
Urotensinas
/
Células Endoteliais
/
Adrenomedulina
Limite:
Humans
Idioma:
Zh
Revista:
Di Yi Jun Yi Da Xue Xue Bao
Assunto da revista:
MEDICINA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
China