A recombinant vaccine against hydatidosis: production of the antigen in Escherichia coli.
J Ind Microbiol Biotechnol
; 33(3): 173-82, 2006 Mar.
Article
em En
| MEDLINE
| ID: mdl-16195870
ABSTRACT
A commercial process was developed for producing a recombinant vaccine against hydatidosis in farm animals. The vaccine antigen consisting of a surface protein of the oncospheres of the hydatid worm (Echinococcus granulosus), was produced as inclusion bodies in Escherichia coli. Fed-batch cultures of E. coli using Terrific broth in stirred bioreactors at 37 degrees C, pH 7.0, and a dissolved oxygen level of 30% of air saturation produced the highest volumetric concentrations of the final solubilized antigen. An exponential feeding strategy proved distinctly superior to feeding based on pH-stat and DO-stat methods. The plasmid coding for the antigen was induced with isopropyl-beta-D-thiogalactopyranoside (IPTG) at 4 h after initiation of the culture. The minimum IPTG concentration for full induction was 0.1 mM.
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Bases de dados:
MEDLINE
Assunto principal:
Echinococcus granulosus
/
Equinococose
/
Antígenos de Helmintos
Limite:
Animals
Idioma:
En
Revista:
J Ind Microbiol Biotechnol
Assunto da revista:
BIOTECNOLOGIA
/
MICROBIOLOGIA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Nova Zelândia