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Genotoxicity of acrylamide and glycidamide in human lymphoblastoid TK6 cells.
Koyama, Naoki; Sakamoto, Hiroko; Sakuraba, Mayumi; Koizumi, Tomoko; Takashima, Yoshio; Hayashi, Makoto; Matsufuji, Hiroshi; Yamagata, Kazuo; Masuda, Shuichi; Kinae, Naohide; Honma, Masamitsu.
Afiliação
  • Koyama N; Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Mutat Res ; 603(2): 151-8, 2006 Feb 28.
Article em En | MEDLINE | ID: mdl-16387526
The recent finding that acrylamide (AA), a potent carcinogen, is formed in foods during cooking raises human health concerns. In the present study, we investigated the genotoxicity of AA and its metabolite glycidamide (GA) in human lymphoblastoid TK6 cells examining three endpoints: DNA damage (comet assay), clastogenesis (micronucleus test) and gene mutation (thymidine kinase (TK) assay). In a 4 h treatment without metabolic activation, AA was mildly genotoxic in the micronucleus and TK assays at high concentrations (> 10 mM), whereas GA was significantly and concentration-dependently genotoxic at all endpoints at > or = 0.5 mM. Molecular analysis of the TK mutants revealed that AA predominantly induced loss of heterozygosity (LOH) mutation like spontaneous one while GA-induced primarily point mutations. These results indicate that the genotoxic characteristics of AA and GA were distinctly different: AA was clastogenic and GA was mutagenic. The cytotoxicity and genotoxicity of AA were not enhanced by metabolic activation (rat liver S9), implying that the rat liver S9 did not activate AA. We discuss the in vitro and in vivo genotoxicity of AA and GA.
Assuntos
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Bases de dados: MEDLINE Assunto principal: Dano ao DNA / Linfócitos / Acrilamida / Compostos de Epóxi Limite: Animals / Humans Idioma: En Revista: Mutat Res Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Japão
Buscar no Google
Bases de dados: MEDLINE Assunto principal: Dano ao DNA / Linfócitos / Acrilamida / Compostos de Epóxi Limite: Animals / Humans Idioma: En Revista: Mutat Res Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Japão