[Effect of acidic fibroblast growth factor on mitogen-activated protein kinase activity in small intestinal epithelium after ischemia/reperfusion injury in rat].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
; 18(1): 9-12, 2006 Jan.
Article
em Zh
| MEDLINE
| ID: mdl-16464375
ABSTRACT
OBJECTIVE:
To investigate the effect of acidic fibroblast growth factor (aFGF) on p44/p42 mitogen-activated protein kinase (p44/p42 MAPK, extracellular signal-regulated protein kinase, ERK1/2) activity in small intestinal epithelium in rat after ischemia/reperfusion (I/R) injury and its relation with the change in small intestinal epithelium in rat after I/R injury as well as the change in proliferation of epithelial cells.METHODS:
Superior mesenteric artery (SMA) was occluded to produce ischemia of the intestine for 45 minutes followed by reperfusion to reproduce I/R injury. One hundred and thirty-two Wistar rats were randomly divided into sham-operation group, I/R group, aFGF treatment group (4 microg of aFGF was injected into jugular vein after reperfusion), and PD98059 (antagonist of ERK 1/2) pretreatment group (7.5 microg of PD98059 was injected via tail vein before ischemia and 4 microg of aFGF was injected via jugular vein after reperfusion). The activity of ERK1/2 and proliferation rate of small intestinal epithelial cells were determined before ischemia and 15 minutes, 30 minutes, 1 hour, 2 hours, 6 hours, 12 hours and 24 hours after reperfusion.RESULTS:
The activity of ERK1/2 in small intestinal epithelium was higher in aFGF treatment group than in I/R control group, and the proliferation rate in small intestinal epithelial cells was significantly enhanced in aFGF treatment group. PD98059, the specific inhibitor of ERK1/2, inhibited ERK1/2 activity and reduced the proliferation rate of small intestinal epithelial cells in aFGF treatment group.CONCLUSION:
aFGF can promote small intestinal epithelial cell proliferation in I/R injury rats, and this may be related to activation of ERK1/2 in small intestinal epithelium.
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Bases de dados:
MEDLINE
Assunto principal:
Traumatismo por Reperfusão
/
Fator 1 de Crescimento de Fibroblastos
/
Proteínas Quinases Ativadas por Mitógeno
/
Mucosa Intestinal
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
Assunto da revista:
MEDICINA DE EMERGENCIA
/
TERAPIA INTENSIVA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
China