Deguelin inhibits expression of IkappaBalpha protein in Raji and U937 cells.
Acta Pharmacol Sin
; 27(4): 485-90, 2006 Apr.
Article
em En
| MEDLINE
| ID: mdl-16539850
ABSTRACT
AIM:
To determine whether deguelin can regulate the expression of nuclear factor kappa B (NF-kappaB) binding protein (IkappaBalpha) in U937 human leukemia cells and Raji human B lymphoma cells.METHODS:
The localization of IkappaBalpha protein was investigated by using an immunofluorescence method. The expression of IkappaBalpha and NF-kappaB /p65 proteins in Raji and U937 cells were investigated by using Western blotting. Apoptosis was detected through annexin V/PI double-labeled cytometry.RESULTS:
IkappaBalpha localized in the cytoplasm in untreated and deguelin-treated cells. After treatment with tumor necrosis factor alpha (TNF-alpha) or deguelin plus TNF-alpha for 15 min, there was a substantial reduction in the amount of IkappaBalpha protein. The expression of IkappaBalpha was downregulated by deguelin in Raji and U937 cells. Deguelin induced apoptosis in U937 cells.CONCLUSION:
Deguelin inhibited the expression of IkappaBalpha protein in U937 and Raji cells. The anti-proliferative activity of deguelin is related to the signal pathway of NF-kappaB.
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Bases de dados:
MEDLINE
Assunto principal:
Rotenona
/
Linfoma de Células B
/
Apoptose
/
Proteínas I-kappa B
Limite:
Humans
Idioma:
En
Revista:
Acta Pharmacol Sin
Assunto da revista:
FARMACOLOGIA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
China