Ontogeny of beta-adrenergic receptor-mediated cyclic AMP generating system in primary cultured neurons.
Int J Dev Neurosci
; 9(4): 347-56, 1991.
Article
em En
| MEDLINE
| ID: mdl-1659119
ABSTRACT
The developmental changes in the beta-adrenergic receptor/cyclic AMP generating system were examined using mouse cerebral cortical neurons in primary culture. During neuronal growth in vitro, the number of binding sites for [3H]dihydroalprenolol (DHA) showed a tendency to increase (Bmax), while the affinity (Kd) for [3H]DHA did not show any noticeable changes. Basal and isoproterenol-stimulated adenylate cyclase activities as well as the activation of adenylate cyclase by 5'-guanylylimidodiphosphate (GppNHp), NaF and forskolin showed progressive and parallel increases during neuronal growth on a polylysine-coated surface. The treatment of primary cultured neurons with islet-activating protein (IAP), one of the pertussis toxins, attenuated the inhibitory effect of carbachol, a muscarinic agonist, on isoproterenol-induced activation of adenylate cyclase activity. These results indicate that primary cultured neurons possess a cyclic AMP generating system coupled with beta-adrenergic and muscarinic receptors, which is regulated via stimulatory and inhibitory GTP-binding proteins, respectively. The results described above also suggest that the beta-adrenergic receptor, stimulatory and inhibitory types of GTP-binding proteins and adenylate cyclase may develop in a parallel fashion during neuronal growth on a polylysine-coated surface.
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Bases de dados:
MEDLINE
Assunto principal:
Encéfalo
/
Adenilil Ciclases
/
Receptores Adrenérgicos beta
/
AMP Cíclico
/
Di-Hidroalprenolol
/
Isoproterenol
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
Int J Dev Neurosci
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Japão