Abnormalities of chromosome 17 in oesophageal cancer.
J Clin Pathol
; 60(9): 990-4, 2007 Sep.
Article
em En
| MEDLINE
| ID: mdl-17046844
ABSTRACT
BACKGROUND:
Oesophageal cancer is the most common malignancy encountered in South African males, especially in the Eastern Cape and surrounding region of South Africa. There are a number of risk factors and predisposing conditions that have been implicated in the aetiology of the disease. The tylosis oesophageal cancer (TOC) gene, localised to a small region on chromosome 17q25, has been shown to be associated with oesophageal squamous cell carcinoma.AIM:
To investigate loss of heterozygosity (LOH) and microsatellite instability (MSI) in the region of the TOC locus.METHODS:
In 74 oesophagectomy specimens for squamous cell carcinoma, microsatellite PCR was performed using five fluorescently labelled TOC markers. The PCR products were analysed and the data correlated with clinicopathological findings.RESULTS:
LOH ranged from 25% to 60%. LOH for the individual markers was as follows D17S1839, 25%; D17S1864, 36%; D17S1817, 38%; D17S785, 47.8%; and D17S579, 60%. MSI ranged from 4.1% to 6.8% for the five loci in the 17q region. MSI was 4.1% for the markers D17S579, D17S785 and D17S1817. Marker D17S1864 showed MSI to occur in 4 cases (5.4%) and marker D17S1839 in 5 cases (6.8%).CONCLUSION:
No significant relationship between genetic and clinical parameters was observed; however, aberrations in poorly differentiated tumours were high for markers D17S579 and D17S1864 (25% and 37%, respectively), indicating that these markers may have an underlying role in the molecular pathogenesis of oesophageal squamous cell carcinoma. In addition, 63% of patients who died showed LOH for the markers D17S579, D17S1864 and D17S1817.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Cromossomos Humanos Par 17
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Neoplasias Esofágicas
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Carcinoma de Células Escamosas
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Perda de Heterozigosidade
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Instabilidade de Microssatélites
Tipo de estudo:
Risk_factors_studies
Limite:
Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
J Clin Pathol
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
África do Sul