Biochemical evidence of a physical interaction between Sulfolobus solfataricus B-family and Y-family DNA polymerases.
Extremophiles
; 11(2): 277-82, 2007 Mar.
Article
em En
| MEDLINE
| ID: mdl-17082970
ABSTRACT
The hyper-thermophilic archaeon Sulfolobus solfataricus possesses two functional DNA polymerases belonging to the B-family (Sso DNA pol B1) and to the Y-family (Sso DNA pol Y1). Sso DNA pol B1 recognizes the presence of uracil and hypoxanthine in the template strand and stalls synthesis 3-4 bases upstream of this lesion ("read-ahead" function). On the other hand, Sso DNA pol Y1 is able to synthesize across these and other lesions on the template strand. Herein we report evidence that Sso DNA pol B1 physically interacts with DNA pol Y1 by surface plasmon resonance measurements and immuno-precipitation experiments. The region of DNA pol B1 responsible for this interaction has been mapped in the central portion of the polypeptide chain (from the amino acid residue 482 to 617), which includes an extended protease hyper-sensitive linker between the N- and C-terminal modules (amino acid residues Asn482-Ala497) and the alpha-helices forming the "fingers" sub-domain (alpha-helices R, R' and S). These results have important implications for understanding the polymerase-switching mechanism on the damaged template strand during genome replication in S. solfataricus.
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Bases de dados:
MEDLINE
Assunto principal:
Proteínas Arqueais
/
Genoma Arqueal
/
Sulfolobus solfataricus
/
DNA Polimerase Dirigida por DNA
/
Replicação do DNA
Idioma:
En
Revista:
Extremophiles
Assunto da revista:
BIOLOGIA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Itália