ADAM19 is tightly associated with constitutive Alzheimer's disease APP alpha-secretase in A172 cells.
Biochem Biophys Res Commun
; 352(1): 111-7, 2007 Jan 05.
Article
em En
| MEDLINE
| ID: mdl-17112471
ABSTRACT
To elucidate whether new proteases are involved in the processing of amyloid precursor protein (APP), we examined catalytically active ADAM12 and ADAM19 as candidates alpha-secretases. The overexpression of ADAM19 in HEK293 cells resulted in an increase in sAPPalpha. Therefore, we suggest that ADAM19 has a constitutive alpha-secretase activity. We examined regulated alpha-secretase activity by adding phorbol 12-myristate 13-acetate (PMA), but no regulated activity was found. To verify that endogenous ADAM19 has an APP alpha-secretase activity, we examined whether the constitutive level of alpha-secretase activity was reduced by RNA interference with ADAM19 in A172 cells. The amount of secreted sAPPalpha decreased by about 21% following RNAi. These results suggest that ADAM19 has a constitutive alpha-secretase activity for APP.
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Bases de dados:
MEDLINE
Assunto principal:
Proteínas ADAM
/
Secretases da Proteína Precursora do Amiloide
/
Doença de Alzheimer
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Japão