DMPO-OH radical formation from 5,5-dimethyl-1-pyrroline N-oxide (DMPO) in hot water.
Anal Sci
; 23(2): 219-21, 2007 Feb.
Article
em En
| MEDLINE
| ID: mdl-17297236
ABSTRACT
When an aqueous solution of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) was heated at 70 degrees C for 30 min, formation of DMPO-OH was observed by ESR. This DMPO-OH radical formation was suppressed under an argon atmosphere. When water was replaced with ultra-pure water for ICP-MS experiments, DMPO-OH radical formation was also diminished. Under an argon atmosphere in ultra-pure water, the intensity of the DMPO-OH signal decreased to about 1/20 of that observed under aerobic conditions with regular purified water. The addition of hydroxyl radical scavengers such as mannitol did not affect the formation of DMPO-OH, but the signal turned faint in the presence of EDTA. We suggest that DMPO reacted with dissolved oxygen to form DMPO-OH.
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Bases de dados:
MEDLINE
Assunto principal:
Água
/
Sequestradores de Radicais Livres
/
Radical Hidroxila
/
Óxidos N-Cíclicos
Idioma:
En
Revista:
Anal Sci
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Japão