The Arabidopsis nitrate transporter AtNRT2.1 is targeted to the root plasma membrane.
Plant Physiol Biochem
; 45(8): 630-5, 2007 Aug.
Article
em En
| MEDLINE
| ID: mdl-17583518
ABSTRACT
Arabidopsis AtNRT2.1 protein is the best characterized high affinity nitrate transporter in higher plants. However, nothing is known about its sub-cellular localization. In this work, we used GFP imaging to follow the targeting of the AtNRT2.1 protein to the different cell membranes. A polyclonal antibody was also raised against a peptide derived from the AtNRT2.1 sequence. Comparison of wild type and mutant plant extracts showed that this antibody recognized specifically the AtNRT2.1 protein. Microsomal membranes were fractionated on sucrose gradients and immunological detections were performed on the different fractions. Altogether, our results demonstrate that the AtNRT2.1 protein is located in the plasma membrane of the root cells.
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Bases de dados:
MEDLINE
Assunto principal:
Membrana Celular
/
Arabidopsis
/
Raízes de Plantas
/
Proteínas de Transporte de Ânions
/
Proteínas de Arabidopsis
Idioma:
En
Revista:
Plant Physiol Biochem
Assunto da revista:
BIOQUIMICA
/
BOTANICA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
França