Large scale expansion of gamma 9 delta 2 T lymphocytes: Innacell gamma delta cell therapy product.
J Immunol Methods
; 326(1-2): 63-75, 2007 Sep 30.
Article
em En
| MEDLINE
| ID: mdl-17716681
ABSTRACT
gamma9delta2 T lymphocytes are non-conventional lymphocytes presenting a direct cytotoxic effect against a broad range of tumour targets. These cells also secrete inflammatory cytokines that can boost the other components of the immune system. In contrast to conventional CD8(+) T cells, the cytotoxic effect of gamma9delta2 T lymphocytes does not depend on the expression of major histocompatibility complex molecules by target tumour cells. INNACELL gammadeltatrade mark is a cell therapy product obtained by ex vivo amplification of mononuclear cells. The stimulation is achieved by a specific synthetic agonist of gamma9delta2 T lymphocytes, bromohydrin pyrophosphate (BrHPP). After a single stimulation with BrHPP, gamma9delta2 T lymphocytes are expanded for 2 weeks in a closed system in culture medium with interleukin-2 (IL-2). On day 15, cells are washed and harvested in 4% human serum albumin. In this manufacturing process, the total cell population is expanded by approximately 10-fold and gamma9delta2 T lymphocytes undergo a specific 1000-fold expansion, corresponding to a gamma9delta2 T lymphocyte enrichment of more than 70% at the end of the culture. This manufacturing process is much simpler than most current cellular therapy approaches using conventional CD8(+) T-cell lines or clones there is no final or initial separation, no purification step and no use of feeder cells; the specific T-cell receptor-mediated signal provided by BrHPP is sufficient to trigger the IL-2-dependent expansion of the gamma9delta2 subset, which then becomes predominant in the cell culture in large amounts.
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Bases de dados:
MEDLINE
Assunto principal:
Imunoterapia Adotiva
/
Subpopulações de Linfócitos T
/
Receptores de Antígenos de Linfócitos T gama-delta
/
Proliferação de Células
Limite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
França