[A quantitative study on the relationship between the specific binding of glucocorticoid receptor and glucocorticoid biopotency in rat thymocytes].
Sheng Li Xue Bao
; 43(3): 243-8, 1991 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-1788558
ABSTRACT
The inhibitory effect of dexamethasone (Dex) on the incorporation of [3H] Urd and specific binding of [3H] Dex with glucocorticoid receptor (GR) were measured in order to quantitatively study the relationship between these two indices in rat thymocytes. The results showed that there existed a close correlation between the two indices in the range of 10(-9)-10(-5) mol/L Dex. When thymocytes were treated with RU486 in the concentration range of 10(-10)-10(-6) mol/L, both [3H] Dex specific binding and inhibitory effect of Dex were blocked with different fractions. The specific binding was linearly related to Dex biopotency. When extrapolated 100% of specific binding corresponded to 100% of biopotency while 20% of specific binding corresponded approximately to 0% of biopotency. It is likely that GR exhibited an occupancy threshold of about 20% total receptors, with only few spare ones in rat thymocytes.
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Bases de dados:
MEDLINE
Assunto principal:
Dexametasona
/
Linfócitos T
/
Receptores de Glucocorticoides
Limite:
Animals
Idioma:
Zh
Revista:
Sheng Li Xue Bao
Ano de publicação:
1991
Tipo de documento:
Article