Adipose differentiation of bone marrow-derived mesenchymal stem cells using Pluronic F-127 hydrogel in vitro.
Biomaterials
; 29(5): 573-9, 2008 Feb.
Article
em En
| MEDLINE
| ID: mdl-17980905
ABSTRACT
Due to increasing clinical demand for adipose tissue, a suitable scaffold for engineering adipose tissue constructs is needed. In this study, we have developed a three-dimensional (3-D) culture system using bone marrow-derived mesenchymal stem cells (BM-MSC) and a Pluronic F-127 hydrogel scaffold as a step towards the in vitro tissue engineering of fat. BM-MSC were dispersed into a Pluronic F-127 hydrogel with or without type I collagen added. The adipogenic differentiation of the BM-MSC was assessed by cellular morphology and further confirmed by Oil Red O staining. The BM-MSC differentiated into adipocytes in Pluronic F-127 in the presence of adipogenic stimuli over a period of 2 weeks, with some differentiation present even in absence of such stimuli. The addition of type I collagen to the Pluronic F-127 caused the BM-MSC to aggregate into clumps, thereby generating an uneven adipogenic response, which was not desirable.
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Bases de dados:
MEDLINE
Assunto principal:
Células da Medula Óssea
/
Diferenciação Celular
/
Tecido Adiposo
/
Hidrogel de Polietilenoglicol-Dimetacrilato
/
Poloxâmero
/
Células-Tronco Mesenquimais
Limite:
Animals
Idioma:
En
Revista:
Biomaterials
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Austrália