Your browser doesn't support javascript.
loading
[Construction of an in vitro equivalent of the pigmented skin].
Yang, Zhuang-qun; Wang, Zheng-hui; Tu, Jun-bo; Zhang, Tie-liang; Hu, Xiao-yi; Li, Guo-guang; Song, Yong.
Afiliação
  • Yang ZQ; Department of Oral and Maxillofacial Plastic Surgery, Stomatological Hospital, Xi'an Jiaotong University, Xi'an 710004, China. yzhqun@mail.xjtu.edu.cn
Nan Fang Yi Ke Da Xue Xue Bao ; 27(11): 1670-3, 2007 Nov.
Article em Zh | MEDLINE | ID: mdl-18024286
OBJECTIVE: To construct an in vitro equivalent of the pigmented skin using tissue engineering methods. METHODS: Surgically removed foreskins was used as the source of keratinocytes and melanocytes harvested by routine tissue digestion. The fibroblasts were enriched by tissue block culture and seeded into the scaffold constructed using mouse tail collagens to construct the pigmented skin equivalent model. The general structure and the melanocyte distribution and growth status in this model were observed with HE staining and Fontana Masson staining. The ultrastructure of the constructed pigmented skin equivalent was observed by transmission electron microscope. RESULTS AND CONCLUSION: The pigmented skin equivalent model was structurally intact, and allowed optimal cell growth. Fontana Masson staining identified in the basal layer numerous melanocytes in normal growth, and the constructed model was structurally similar to normal skin tissue, suggesting successful construction of the pigmented skin equivalent model.
Assuntos
Buscar no Google
Bases de dados: MEDLINE Assunto principal: Pigmentação da Pele / Pele Artificial / Engenharia Tecidual Limite: Animals / Humans / Male Idioma: Zh Revista: Nan Fang Yi Ke Da Xue Xue Bao Ano de publicação: 2007 Tipo de documento: Article País de afiliação: China
Buscar no Google
Bases de dados: MEDLINE Assunto principal: Pigmentação da Pele / Pele Artificial / Engenharia Tecidual Limite: Animals / Humans / Male Idioma: Zh Revista: Nan Fang Yi Ke Da Xue Xue Bao Ano de publicação: 2007 Tipo de documento: Article País de afiliação: China