Activating transcription factor 3 up-regulated by c-Jun NH(2)-terminal kinase/c-Jun contributes to apoptosis induced by potassium deprivation in cerebellar granule neurons.
Neuroscience
; 151(3): 771-9, 2008 Feb 06.
Article
em En
| MEDLINE
| ID: mdl-18178318
ABSTRACT
Cerebellar granule neurons (CGNs) depend on potassium depolarization for survival and undergo apoptosis when deprived of depolarizing concentration of potassium. Activating transcription factor 3 (ATF3), a stress-inducible protein, belongs to the ATF/CREB family of transcription factors family and is involved in cell growth and apoptosis. However, the role of ATF3 in neuronal apoptosis remains unknown. Here, we showed that ATF3 was up-regulated under potassium deprivation in CGNs, and this induction was preceded by a rapid and sustained activation of c-Jun NH(2)-terminal kinase/c-Jun signaling pathway, which plays a fundamental role in neuronal apoptosis. Furthermore, ATF3 up-regulation was abolished by inhibition of JNK or knockdown of c-Jun. Finally, knockdown of ATF3 by RNA interference protected CGNs from potassium deprivation-induced apoptosis. Taken together, our results indicate that ATF3 is a downstream target of JNK/c-Jun pathway and contributes to apoptosis induced by potassium deprivation in rat CGNs.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Deficiência de Potássio
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Regulação para Cima
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Cerebelo
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Apoptose
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Proteínas Quinases JNK Ativadas por Mitógeno
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Fator 3 Ativador da Transcrição
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Neurônios
Limite:
Animals
Idioma:
En
Revista:
Neuroscience
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
China