Cohesin mediates transcriptional insulation by CCCTC-binding factor.
Nature
; 451(7180): 796-801, 2008 Feb 14.
Article
em En
| MEDLINE
| ID: mdl-18235444
ABSTRACT
Cohesin complexes mediate sister-chromatid cohesion in dividing cells but may also contribute to gene regulation in postmitotic cells. How cohesin regulates gene expression is not known. Here we describe cohesin-binding sites in the human genome and show that most of these are associated with the CCCTC-binding factor (CTCF), a zinc-finger protein required for transcriptional insulation. CTCF is dispensable for cohesin loading onto DNA, but is needed to enrich cohesin at specific binding sites. Cohesin enables CTCF to insulate promoters from distant enhancers and controls transcription at the H19/IGF2 (insulin-like growth factor 2) locus. This role of cohesin seems to be independent of its role in cohesion. We propose that cohesin functions as a transcriptional insulator, and speculate that subtle deficiencies in this function contribute to 'cohesinopathies' such as Cornelia de Lange syndrome.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
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Transcrição Gênica
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Proteínas Nucleares
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Proteínas Cromossômicas não Histona
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Regulação da Expressão Gênica
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Proteínas de Ciclo Celular
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Proteínas de Ligação a DNA
Limite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Nature
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Áustria