Quantifying bluetongue virus in adult Culicoides biting midges (Diptera: Ceratopogonidae).
J Med Entomol
; 45(1): 129-32, 2008 Jan.
Article
em En
| MEDLINE
| ID: mdl-18283953
ABSTRACT
A TissueLyser system (QIAGEN) was used to rapidly and accurately estimate bluetongue virus "loads" in individual adult Culicoides sonorensis Wirth & Jones (Diptera Ceratopogonidae). The optimized homogenization program that was developed, involved shaking insects for 1 min at 25 Hz with 2- or 3-mm stainless steel ball bearings. This program was used to measure the quantities of bluetongue virus present in insects that had either been inoculated or had ingested a viremic bloodmeal through an artificial membrane. The virus titers obtained using either infection technique and the optimized program did not differ significantly from those obtained using a polypropylene motor-driven pestle, a method that is currently in common use for studies of vector competence). The advantages of the new method, as a rapid means of detecting fully disseminated infections in individual field-caught flies, are discussed. Its use is compared with the processing of pools of Culicoides by conventional methods, where the extent of dissemination of the virus is unknown and could wrongly implicate species that are of low importance in transmission.
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Bases de dados:
MEDLINE
Assunto principal:
Ceratopogonidae
/
Vírus Bluetongue
Limite:
Animals
Idioma:
En
Revista:
J Med Entomol
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Reino Unido