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VEGF concentration from plasma-activated platelets rich correlates with microvascular density and grading in canine mast cell tumour spontaneous model.
Patruno, R; Arpaia, N; Gadaleta, C D; Passantino, L; Zizzo, N; Misino, A; Lucarelli, N M; Catino, A; Valerio, P; Ribatti, D; Ranieri, G.
Afiliação
  • Patruno R; Department of Animal Health and Well-Being, University of Bari Veterinary Medical School, Bari, Italy.
J Cell Mol Med ; 13(3): 555-61, 2009 Mar.
Article em En | MEDLINE | ID: mdl-18429933
Canine cutaneous mast cell tumour (CMCT) is a common cutaneous tumour in dog, with a higher incidence than in human. CMCT is classified in three subgroups, well and intermediately differentiated (G1 and G2), corresponding to a benign disease, and poorly differentiated (G3), corresponding to a malignant disease, which metastasize to lymph nodes, liver, spleen and bone marrow. In this study, we have evaluated serum (S), platelet-poor plasma (P-PP), plasma-activated platelet rich (P-APR) and cytosol vascular endothelial growth factor (VEGF) concentrations, microvascular density (MVD) and mast cell density (MCD) in a series of 86 CMCTs and we have correlated these parameters with each other, by means of ELISA detection of VEGF and immunohistochemistry. Results show that VEGF level from cytosol P-APR and MVD were significantly higher in G3 CMCTs as compared to G1 or G2 subgroups. Moreover, a significantly strong correlation among VEGF levels from P-PAR and cytosol, MVD and MCD was found in G3 subgroup. Because VEGF levels from P-APR well correlated with MVD and malignancy grade in CMCT, we suggest that VEGF might be secreted from MCs and it may be a suitable surrogate inter-species angiogenetic markers of tumour progression in CMCT. Finally, CMCT seems to be a useful model to study the role of MCs in tumour angiogenesis and inhibition of MCs degranulation or activation might be a new anti-angiogenic strategy worthy to further investigations.
Assuntos

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Mastocitose / Fator A de Crescimento do Endotélio Vascular / Doenças do Cão / Plasma Rico em Plaquetas / Microvasos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Cell Mol Med Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Itália

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Mastocitose / Fator A de Crescimento do Endotélio Vascular / Doenças do Cão / Plasma Rico em Plaquetas / Microvasos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Cell Mol Med Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Itália