Identification of novel chicken microRNAs and analysis of their genomic organization.
Gene
; 418(1-2): 34-40, 2008 Jul 15.
Article
em En
| MEDLINE
| ID: mdl-18511220
ABSTRACT
MicroRNAs (miRNAs) represent a family of small noncoding RNAs with important regulatory roles in diverse biological processes ranging from cell differentiation to organism development. In chickens, the full set of miRNAs and the expression patterns of miRNAs during development are still poorly understood when compared to the other vertebrates. In this study, we identified 29 novel miRNAs and 140 potential miRNA loci in the chicken genome by combining the experimental and computational analyses. Detailed expression patterns of 49 miRNAs were first characterized by Northern blotting and indicated the cooperativity of the miRNA expression with their function in embryogenesis and organogenesis. Twenty-seven miRNA clusters were systematically evaluated in the chicken genome and diverse expression patterns for closely linked miRNAs were observed. Our results significantly expand the set of known miRNAs in the chicken and provide the basis for understanding the structural and functional evolution of miRNA genes in vertebrates.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Galinhas
/
Mapeamento Cromossômico
/
MicroRNAs
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Gene
Ano de publicação:
2008
Tipo de documento:
Article