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Multivesicular bodies in intestinal epithelial cells: responsible for MHC class II-restricted antigen processing and origin of exosomes.
Büning, Jürgen; von Smolinski, Dorthe; Tafazzoli, Kianush; Zimmer, Klaus-Peter; Strobel, Stephan; Apostolaki, Maria; Kollias, George; Heath, Joan K; Ludwig, Diether; Gebert, Andreas.
Afiliação
  • Büning J; Department of Internal Medicine I, University Hospital of Schleswig-Holstein, Lübeck, Germany. juergen.buening@uk-sh.de
Immunology ; 125(4): 510-21, 2008 Dec.
Article em En | MEDLINE | ID: mdl-18710406
In normal conditions intestinal epithelial cells (IECs) constitutively stimulate regulatory CD4(+) T cells. However, in Crohn's disease (CD), this major histocompatibility complex (MHC) class II-restricted antigen presentation results in stimulation of proinflammatory CD4(+) T cells. We hypothesized that these alternative functions might be mediated by differential sorting and processing of antigens into distinct MHC II-enriched compartments (MIICs). Accordingly, we analysed the endocytic pathways of lumenally applied ovalbumin (OVA) in IECs of the jejunum and ileum of wild-type (WT) and TNFDeltaARE/WT mice that develop a CD-resembling ileitis. Using quantitative reverse transcription polymerase chain reaction, we found that messenger RNA levels of interferon-gamma, tumour necrosis factor-alpha, interleukin-17 and interleukin-10 were significantly up-regulated in the inflamed ileum of TNFDeltaARE/WT mice, confirming CD-like inflammation. Fluorescence and immunoelectron microscopy revealed the presence of MHC II and invariant chain throughout the late endocytic compartments, with most molecules concentrated in the multivesicular bodies (MVB). OVA was targeted into MVB and, in contrast to other MIICs, accumulated in these structures within 120 min of exposure. The IEC-specific A33 antigen localized to internal vesicles of MVB and A33/class II-bearing exosomes were identified in intercellular spaces. Remarkably, the expression pattern of MHC II/invariant chain molecules and the trafficking of OVA were independent of mucosal inflammation and the specific region in the small intestine. MVB seem to be principally responsible for class II-associated antigen processing in IECs and to constitute the origin of MHC II-loaded exosomes. The distinctive functions of IECs in antigen presentation to CD4(+) T cells might arise as a result of differential processing within the MVB identified here.
Assuntos

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Endossomos / Antígenos de Histocompatibilidade Classe II / Exossomos / Mucosa Intestinal Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Immunology Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Endossomos / Antígenos de Histocompatibilidade Classe II / Exossomos / Mucosa Intestinal Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Immunology Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Alemanha