Embryonic stem cells derived from C57BL/6J and C57BL/6N mice.
Comp Med
; 58(4): 347-52, 2008 Aug.
Article
em En
| MEDLINE
| ID: mdl-18724776
ABSTRACT
Mouse embryonic stem (ES) cells with the C57BL/6 genetic background allow the generation of knockout mice without the need to backcross to C57BL/6. However, C57BL/6 ES cells whose pluripotency after homologous recombination has been confirmed are not yet available from public cell banks. To facilitate the use of ES cells derived from C57BL/6 sublines in both biologic and medical research, we demonstrated that the use of knockout serum replacement as a medium supplement and 8-cell blastomeres as recipient embryos allowed establishment of ES cells and production of germline chimeric mice, respectively. Under effective conditions, a large number of ES cell lines were established from C57BL/6J and C57BL/6N blastocysts. The majority of ES cells in many cell lines obtained from both strains showed a normal chromosome number. Germline chimeric mice were generated from C57BL/6J and C57BL/6N ES cells. Finally, the ES cell line B6J-S1UTR, derived from C57BL/6J, was used for successful production of gene knockout mice. C57BL/6J ES (B6J-S1UTR and B6J-23UTR) and C57BL/6N ES (B6N-22UTR) cells are available from the cell bank of the BioResource Center at RIKEN Tsukuba Institute (http//www.brc.riken.jp/lab/cell/english/).
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Células-Tronco Embrionárias
/
Camundongos Endogâmicos C57BL
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Comp Med
Assunto da revista:
MEDICINA
/
MEDICINA VETERINARIA
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Japão