Quantification of L-abrine in human and rat urine: a biomarker for the toxin abrin.
J Anal Toxicol
; 33(2): 77-84, 2009 Mar.
Article
em En
| MEDLINE
| ID: mdl-19239732
ABSTRACT
Abrin is a toxic protein found in the jequirity seed. L-Abrine (N-methyl-tryptophan) is also found in the jequirity seed and can be used as a biomarker for abrin exposure. Analysis of L-abrine was added to an existing method for quantifying ricinine as a marker for ricin exposure in human urine and analytically validated. Accuracy and reproducibility were enhanced by including a newly synthesized (13)C(1)(2)H(3)-L-abrine internal standard. One-milliliter urine samples were processed using solid-phase extraction prior to a 6-min high-performance liquid chromatography separation. Protonated molecular ions were formed via electrospray ionization in a triple-quadrupole mass spectrometer and quantified via multiple reaction monitoring. Method validation included the characterization of two enriched urine pools, which were used as quality control materials. Endogenous levels of L-abrine were quantified in a reference range of 113 random urine samples at 0.72 +/- 0.51 ng/mL. Urinary concentrations of L-abrine were monitored in an intentional rat exposure study for up to 48 h. Comparing the results from the human reference range and the animal exposure study indicates that this method is suitable for quantifying L-abrine within 24 h post-exposure. Quantification of L-abrine beyond 24 h is limited by rapid excretion of the biomarker and the level of the L-abrine dose.
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Bases de dados:
MEDLINE
Assunto principal:
Alcaloides Indólicos
/
Abrina
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
J Anal Toxicol
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos