Isolation and characterization of metal-binding proteins (metallothioneins) from lobster digestive gland (Homarus americanus).
Sci Total Environ
; 105: 41-59, 1991 Jun.
Article
em En
| MEDLINE
| ID: mdl-1925523
ABSTRACT
Two metallothionein (low-molecular-weight, metal-binding proteins) preparations, MT-1 and MT-2, have been isolated from the digestive gland of American lobster (Homarus americanus) contaminated with Cd. MT-1 contains Cd- and Cu-binding proteins, whereas MT-2 is a reasonably pure Cd-binding protein. The properties of MT-1 and MT-2 with respect to amino acid and elemental compositions, heat stabilities, polarographic, high-performance liquid chromatography (HPLC), and isoelectric focussing behaviors are reported. Lobster metallothioneins share a number of similarities with mammalian metallothioneins with respect to the presence of Cd and Cu, apparent molecular weights, amino acid compositions, UV absorption spectra at various pH, and polarographic behavior, but differ substantially in their electrophoretic behavior.
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Bases de dados:
MEDLINE
Assunto principal:
Cádmio
/
Sistema Digestório
/
Metalotioneína
Limite:
Animals
Idioma:
En
Revista:
Sci Total Environ
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Canadá