O-GlcNAcylation of Sp1 interrupts Sp1 interaction with NF-Y.
Biochem Biophys Res Commun
; 382(3): 593-7, 2009 May 08.
Article
em En
| MEDLINE
| ID: mdl-19302979
ABSTRACT
O-linked N-acetylglucosamine (O-GlcNAc), a monosaccharide N-acetylglucosamine addition on nucleocytoplasmic proteins, is abundant in transcription regulators and has been implicated in gene regulation. Sp1 transcription factor is multiply modified by O-GlcNAc within its serine/threonine-rich region and glutamine-rich transactivation domain. In the present study, we show that O-GlcNAc of Sp1 serine/threonine-rich region interrupts a physical interaction between Sp1 and NF-YA, thus inhibiting Sp1-NF-Y cooperative activation of gene transcription. Our results strengthen the notion that O-GlcNAc regulates gene transcription by modulating the protein-protein interaction network among transcription regulatory proteins.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Acetilglucosamina
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Ativação Transcricional
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Fator de Transcrição Sp1
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Fator de Ligação a CCAAT
Limite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2009
Tipo de documento:
Article