Highly Efficient ENU Mutagenesis in Zebrafish.
Methods Mol Biol
; 546: 3-12, 2009.
Article
em En
| MEDLINE
| ID: mdl-19378094
ABSTRACT
ENU (N-ethyl-N-nitrosourea) mutagenesis is a widely accepted and proven method to introduce random point mutations in the genome. Because there are no targeted knockout strategies available for zebrafish so far, random mutagenesis is currently the preferred method in both forward and reverse genetic approaches. To obtain high-density mutagenized zebrafish, six consecutive ENU treatments are applied at weekly intervals to adult male zebrafish by bathing them in ENU solution. With this procedure an average germ line mutation load of one mutation every 1.0 x 10(5)-1.5 x 10(5) basepairs is reached routinely in our lab.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Peixe-Zebra
/
Mutagênese
/
Etilnitrosoureia
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Holanda