High-throughput genotyping of KIR2DL2/L3, KIR3DL1/S1, and their HLA class I ligands using real-time PCR.
Tissue Antigens
; 74(1): 73-80, 2009 Jul.
Article
em En
| MEDLINE
| ID: mdl-19522772
ABSTRACT
Killer immunoglobulin-like receptors (KIRs) expressed on natural killer cells are critical components of innate immunity. Interactions between KIRs and their human leukocyte antigen (HLA) ligands have been shown to influence autoimmune and infectious disease course in defined populations. However, the low throughput and high cost of current methods impede confirmation of the universality of these findings. To support large epidemiology surveys, we developed a high-throughput real-time polymerase chain reaction-based assay to identify carriers of KIR3DL1, KIR3DS1, KIR2DL2, and KIR2DL3 and their HLA ligands. The platform performed with 100% sensitivity and specificity in detection of carrier and non-carrier on reference samples. The application of this platform will further clarify the nature and impact of the KIR-HLA epistatic interaction on disease course in large global population-based studies.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Antígenos de Histocompatibilidade Classe I
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Reação em Cadeia da Polimerase
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Receptores KIR2DL2
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Receptores KIR2DL3
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Receptores KIR3DL1
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Receptores KIR3DS1
Tipo de estudo:
Diagnostic_studies
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Evaluation_studies
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Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Tissue Antigens
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos