Transforming growth interacting factor expression in leiomyoma compared with myometrium.
Fertil Steril
; 94(3): 1078-83, 2010 Aug.
Article
em En
| MEDLINE
| ID: mdl-19524896
ABSTRACT
OBJECTIVE:
To investigate the expression of transforming growth interacting factor (TGIF), a Smad transcriptional corepressor, in leiomyoma and matched myometrial tissue samples and the effect of TGIF overexpression in myometrial cells.DESIGN:
Experimental study.SETTING:
Tertiary university hospital. PATIENT(S) Uterine leiomyoma and myometrial tissues from 16 patients. INTERVENTION(S) None. MAIN OUTCOME MEASURE(S) The distribution of TGIF in leiomyoma and myometrial tissues by immunohistochemistry stain, mRNA, and protein expression levels by real-time quantitative polymerase chain-reaction (QPCR) and Western blot. Transcriptional regulation of TGIF in myometrial cells with overexpressed TGIF. RESULT(S) Although TGIF is present in the smooth muscle cells of the leiomyoma and the myometrium, it is not found in the extracellular matrix. The TGIF mRNA and protein expressions were statistically significantly higher in the leiomyoma compared with the matched, unaffected myometrial tissues in both phases of the menstrual cycle. There were no differences in mRNA or protein expression throughout the menstrual cycle. Overexpression of TGIF protein in myometrial cells statistically significantly suppressed up-regulation of plasminogen activator inhibitor (PAI-1) induced by TGF-beta1 treatment. CONCLUSION(S) Expression of TGIF is increased in leiomyoma compared with myometrium. This increase in TGIF expression is not affected by endogenous ovarian hormones. Thus, TGIF is a potential repressor of TGF-beta pathways in myometrial cells.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
/
Neoplasias Uterinas
/
Proteínas de Homeodomínio
/
Leiomioma
/
Miométrio
Limite:
Female
/
Humans
Idioma:
En
Revista:
Fertil Steril
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Estados Unidos