Amino acid substitutions within the 2C coding sequence of Theiler's Murine Encephalomyelitis virus alter virus growth and affect protein distribution.
Virus Res
; 144(1-2): 74-82, 2009 Sep.
Article
em En
| MEDLINE
| ID: mdl-19728403
ABSTRACT
Theiler's murine encephalomyelitis virus (TMEV) was used to investigate the distribution of P2 proteins in host cells and examine the effect of amino acid substitutions in conserved residues of the 2C protein on virus growth. The distribution of viral proteins 2B, 2C and 2BC with marker proteins of the endoplasmic reticulum (ER) and/or Golgi suggest an association with membranes of the secretory pathway. Similar results were obtained for truncated 2C and 2BC proteins with C-terminal deletions suggesting that the N-terminal region of the 2C protein is important in dictating distribution patterns. The significance of the high degree of conservation of this 2C region throughout the Picornaviridae was investigated by substituting conserved amino acid residues for alanine to create six mutant strains. Substitution mutations E(8)A, W(18)A and W(29)A abolished the ability of the virus to induce cytopathic effect (CPE) in BHK-21 cells. K(14)A, R(4)A and I(23)A delayed the onset and progression of CPE compared to the wild-type (WT) virus, and decreased virus yield. Immunofluorescence analysis of cells transiently expressing mutant 2C proteins revealed that the distribution of 2C was affected by substituting K(14), W(18) and I(23) for alanine indicating that specific conserved residues in 2C dictate protein distribution and virus growth.
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Bases de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
Replicação Viral
/
Theilovirus
/
Substituição de Aminoácidos
Limite:
Animals
Idioma:
En
Revista:
Virus Res
Assunto da revista:
VIROLOGIA
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
África do Sul