Expression of Escherichia coli Min system in Bacillus subtilis and its effect on cell division.
FEMS Microbiol Lett
; 302(1): 58-68, 2010 Jan.
Article
em En
| MEDLINE
| ID: mdl-19903201
ABSTRACT
In both rod-shaped Bacillus subtilis and Escherichia coli cells, Min proteins are involved in the regulation of division septa formation. In E. coli, dynamic oscillation of MinCD inhibitory complex and MinE, a topological specificity protein, prevents improper polar septation. However, in B. subtilis no MinE is present and no oscillation of Min proteins can be observed. The function of MinE is substituted by that of an unrelated DivIVA protein, which targets MinCD to division sites and retains them at the cell poles. We inspected cell division when the E. coli Min system was introduced into B. subtilis cells. Expression of these heterologous Min proteins resulted in cell elongation. We demonstrate here that E. coli MinD can partially substitute for the function of its B. subtilis protein counterpart. Moreover, E. coli MinD was observed to have similar helical localization as B. subtilis MinD.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Bacillus subtilis
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Divisão Celular
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Adenosina Trifosfatases
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Proteínas de Ciclo Celular
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Proteínas de Escherichia coli
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Escherichia coli
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Proteínas de Membrana
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Eslováquia