Minocycline inhibits 5-lipoxygenase expression and accelerates functional recovery in chronic phase of focal cerebral ischemia in rats.
Life Sci
; 86(5-6): 170-7, 2010 Jan 30.
Article
em En
| MEDLINE
| ID: mdl-20006627
ABSTRACT
AIMS:
We previously reported that minocycline attenuates acute brain injury and inflammation after focal cerebral ischemia, and this is partly mediated by inhibition of 5-lipoxygenase (5-LOX) expression. Here, we determined the protective effect of minocycline on chronic ischemic brain injury and its relation with the inhibition of 5-LOX expression after focal cerebral ischemia. MAINMETHODS:
Focal cerebral ischemia was induced by 90 min of middle cerebral artery occlusion followed by reperfusion for 36 days. Minocycline (45 mg/kg) was administered intraperitoneally 2h and 12h after ischemia and then every 12h for 5 days. Sensorimotor function was evaluated 1-28 days after ischemia and cognitive function was determined 30-35 days after ischemia. Thereafter, infarct volume, neuron density, astrogliosis, and 5-LOX expression in the brain were determined. KEYFINDINGS:
Minocycline accelerated the recovery of sensorimotor and cognitive functions, attenuated the loss of neuron density, and inhibited astrogliosis in the boundary zone around the ischemic core, but did not affect infarct volume. Minocycline significantly inhibited the increased 5-LOX expression in the proliferated astrocytes in the boundary zone, and in the macrophages/microglia in the ischemic core.SIGNIFICANCE:
Minocycline accelerates functional recovery in the chronic phase of focal cerebral ischemia, which may be partly associated with the reduction of 5-LOX expression.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Encéfalo
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Isquemia Encefálica
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Inibidores de Lipoxigenase
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Fármacos Neuroprotetores
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Minociclina
Tipo de estudo:
Etiology_studies
Idioma:
En
Revista:
Life Sci
Ano de publicação:
2010
Tipo de documento:
Article