Development of positron emission tomography imaging by 64Cu-labeled Fab for detecting ERC/mesothelin in a mesothelioma mouse model.
Nucl Med Commun
; 31(5): 380-8, 2010 May.
Article
em En
| MEDLINE
| ID: mdl-20072072
ABSTRACT
BACKGROUND:
Malignant mesothelioma is a highly aggressive form of cancer. Curative surgery is the only effective therapy for mesothelioma, and therefore early diagnosis is important. However, early diagnosis is difficult using current diagnostic imaging techniques, and a new imaging method for early diagnosis is urgently required. We evaluated the affinity of radiolabeled monoclonal antibodies to the C-terminal fragment of ERC/mesothelin for this purpose.METHODS:
In-labeled or I-labeled IgG against C-terminal fragment of ERC and its Fab fragment were evaluated in vitro by cell binding, competitive inhibition, and cellular internalization assays, and in vivo by biodistribution in mice bearing ERC-expressing tumors. Next, the Fab fragment was labeled with the positron emitter Cu and evaluated by positron emission tomography (PET).RESULTS:
Radiolabeled IgG and Fab showed specific binding to ERC-expressing mesothelioma cells with high affinity. Both radiolabeled IgG and Fab internalized into cells after binding to ERC on the cell surface. In-labeled IgG accumulated in ERC-expressing tumors and resulted in a moderate tumor-to-blood ratio at 4 days after injection. Furthermore, PET using Cu-labeled Fab visualized the tumor at 6 h after injection.CONCLUSION:
Cu-labeled Fab can be useful for ERC-specific PET imaging, and can thus facilitate improved diagnosis of patients with early-stage mesothelioma.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Fragmentos Fab das Imunoglobulinas
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Radioisótopos de Cobre
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Glicoproteínas de Membrana
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Tomografia por Emissão de Pósitrons
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Mesotelioma
Tipo de estudo:
Prognostic_studies
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Screening_studies
Limite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Nucl Med Commun
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Japão