Assessment of embryotoxicity of compounds in cosmetics by the embryonic stem cell test.
Toxicol Mech Methods
; 20(3): 112-8, 2010 Mar.
Article
em En
| MEDLINE
| ID: mdl-20100057
ABSTRACT
The new EU legislations for cosmetics (Seventh Amendment) have laid down deadlines for the replacement of animal tests in cosmetics. This policy stimulates the acceptance of in vitro approaches to test embryotoxic potentials of compounds in cosmetics products. The embryonic stem cell test (EST) designed by The European Centre for the Validation of Alternative Methods (ECVAM) is currently the most promising in vitro assay to predict the embryotoxic potential of compounds. In this study, six selected compounds (hydroquinone, eugenol, dibutyl phthalate, antimony (III) oxide, neodymium (III) nitrate hydrate, melamine) formerly involved in cosmetic products were selected to test their embryotoxic potentials by the EST. 5-Fluorouracil and penicillin G were separately set as positive and negative control. The embryotoxic potential was determined by the prediction model (PM), which was calculated from three endpoints, the IC(50) 3T3, IC(50) ES, and ID(50). Hydroquinone, eugenol, and antimony (III) oxide indicated with strong embryotoxicity, while dibutyl phthalate, neodymium (III) nitrate hydrate, and melamine exhibited a weak embryotoxicity. These results may provide a valuable attempt to expand the application of EST to the cosmetics field.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Teratogênicos
/
Testes de Toxicidade
/
Cosméticos
/
Células-Tronco Embrionárias
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Toxicol Mech Methods
Assunto da revista:
TOXICOLOGIA
Ano de publicação:
2010
Tipo de documento:
Article