Two forms of activation-induced cytidine deaminase differing in their ability to bind agarose.
PLoS One
; 5(1): e8883, 2010 Jan 27.
Article
em En
| MEDLINE
| ID: mdl-20111710
ABSTRACT
BACKGROUND:
Activation-induced cytidine deaminase (AID) is a B-cell-specific DNA mutator that plays a key role in the formation of the secondary antibody repertoire in germinal center B cells. In the search for binding partners, protein coimmunoprecipitation assays are often performed, generally with agarose beads. METHODOLOGY/PRINCIPALFINDINGS:
We found that, regardless of whether cell lysates containing exogenous or endogenous AID were examined, one of two mouse AID forms bound to agarose alone. CONCLUSIONS/SIGNIFICANCE:
These binding characteristics may be due to the known post-translational modifications of AID; they may also need to be considered in coimmunoprecipitation experiments to avoid false-positive results.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Sefarose
/
Citidina Desaminase
/
Isoenzimas
Limite:
Animals
Idioma:
En
Revista:
PLoS One
Assunto da revista:
CIENCIA
/
MEDICINA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Alemanha