Promotion of self-renewal of embryonic stem cells by midkine.
Acta Pharmacol Sin
; 31(5): 629-37, 2010 May.
Article
em En
| MEDLINE
| ID: mdl-20442752
ABSTRACT
AIM:
To characterize the expression and function of midkine (MK) in an in vitro embryonic stem cell (ESC) culture system.METHODS:
To investigate the potential roles of MK, the expression of MK in ESCs was evaluated by RT-PCR and immunocytochemistry. The effects of MK on the self-renewal of ESCs were measured using alkaline phosphatase assays, immunocytochemistry, RT-PCR and colony-forming assays. The mechanism of the growth-promoting effect of MK in mESCs was assessed by cell cycle analysis and Western blot analysis.RESULTS:
MK is expressed in mouse embryonic stem cells (mESCs), human embryonic stem cells (hESCs) and mouse embryonic fibroblasts (MEFs). MK promotes proliferation and self-renewal of mESCs both in feeder and feeder free culture systems. It also promotes self-renewal and proliferation of hESCs. Further study showed that MK promotes the growth of mESCs by inhibiting apoptosis while accelerating the progression toward the S phase, and enhances mESC self-renewal through PI3K/Akt signaling pathway.CONCLUSION:
MK plays profound roles in ESCs. MK/PTPzeta signaling pathway is a novel pathway in the signal network maintaining pluripotency of ESCs. The results extend our knowledge on pluripotency control of ESCs and the relationship between ESCs and cancers.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Citocinas
/
Proliferação de Células
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Células-Tronco Embrionárias
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Acta Pharmacol Sin
Assunto da revista:
FARMACOLOGIA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
China