Dichotomous response to transforming growth factor ß after T cell receptor activation by naive CD4+ T cells from DBA/1 mice: enhanced retinoic acid receptor-related orphan nuclear receptor γt expression yet reduced FoxP3 expression.
Arthritis Rheum
; 63(1): 118-26, 2011 Jan.
Article
em En
| MEDLINE
| ID: mdl-20862680
ABSTRACT
OBJECTIVE:
To investigate the molecular mechanism for biased interleukin-17 (IL-17) production by DBA/1 CD4+ T cells upon T cell receptor (TCR) and transforming growth factor ß (TGFß) stimulation.METHODS:
Purified naive CD4+ T cells were stimulated with anti-CD3/CD28 under Th1, Th2, Th17, and induced T regulatory (iTreg) cell conditions. Cytokine production was assayed by intracellular staining and enzyme-linked immunosorbent assay. Expression of transcription factors was determined by reverse transcription-polymerase chain reaction, flow cytometry, and immunoblotting techniques.RESULTS:
Naive CD4+ T cells from DBA/1 mice produced more IL-17 under Th17 cell polarizing conditions as compared with those from C57BL/6 or BALB/c mice. Further investigation revealed no difference among the strains in terms of CD4+ T cell survival, upstream TCR signaling, or CD69 expression or in the phosphorylation of STAT-3 and expression of suppressor of cytokine signaling 3 that positively or negatively regulate IL-17 cell production. However, DBA/1 CD4+ T cells expressed increased levels of retinoic acid-related orphan receptor γt (RORγt). Furthermore, under iTreg cell polarizing conditions, DBA/1 CD4+ T cells showed a strikingly reduced level of FoxP3 expression. When interferon-γ and IL-4 were blocked, FoxP3 expression increased but remained lower in DBA/1 CD4+ T cells following exposure to TGFß as compared with C57BL/6 CD4+ T cells. Moreover, DBA/1 CD4+ T cells showed reduced phosphorylation of Smad2 and Smad3 under both Th17 and iTreg cell polarizing conditions.CONCLUSION:
These results indicate that naive CD4+ T cells from DBA/1 mice have a dichotomous response to TGFß enhanced RORγt, yet reduced FoxP3, up-regulation. This observation may help to elucidate the branch point of TGFß signaling that leads to skewed Th17, but reduced iTreg, cell differentiation.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Receptores de Antígenos de Linfócitos T
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Linfócitos T CD4-Positivos
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Fatores de Transcrição Forkhead
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Fator de Crescimento Transformador beta1
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Membro 1 do Grupo F da Subfamília 1 de Receptores Nucleares
Limite:
Animals
Idioma:
En
Revista:
Arthritis Rheum
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Estados Unidos