Interaction of DNA with nuclear skeleton in rat hepatocytes.

Widlak, P; Rogolinski, J; Rzeszowska-Wolny, J

Widlak, P; Rogolinski, J; Rzeszowska-Wolny, J.

Afiliação

Widlak P; Institute of Oncology, Department of Tumour Biology, Gliwice, Poland.

Acta Biochim Pol ; 37(1): 69-72, 1990.

Article em En | MEDLINE | ID: mdl-2087918

RESUMO

We have characterized fractions liberated from rat liver cell nuclei digested with DNase I and treated with buffers containing 0.1, 0.5 and 2 M NaCl. Analysis of DNA and proteins present in these fractions as well as in nuclear matrix, confirms the chromatin model according to which transcriptionally active loops interact differently with nuclear skeleton than inactive ones.

Assuntos

DNA/metabolismo; Fígado/metabolismo; Matriz Nuclear/metabolismo; Animais; DNA/genética; DNA/isolamento & purificação; Desoxirribonuclease I; Genes; Oncogenes; Ratos; Sequências Repetitivas de Ácido Nucleico

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Bases de dados: MEDLINE Assunto principal: DNA / Matriz Nuclear / Fígado Limite: Animals Idioma: En Revista: Acta Biochim Pol Ano de publicação: 1990 Tipo de documento: Article País de afiliação: Polônia

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