Cotton plants expressing CYP6AE14 double-stranded RNA show enhanced resistance to bollworms.
Transgenic Res
; 20(3): 665-73, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-20953975
ABSTRACT
RNA interference (RNAi) plays an important role in regulating gene expression in eukaryotes. Previously, we generated Arabidopsis and tobacco plants expressing double-stranded RNA (dsRNA) targeting a cotton bollworm (Helicoverpa armigera) P450 gene, CYP6AE14. Bollworms fed on transgenic dsCYP6AE14 plants showed suppressed CYP6AE14 expression and reduced growth on gossypol-containing diet (Mao et al., in Nat Biotechnol 25 1307-1313, 2007). Here we report generation and analysis of dsRNA-expressing cotton (Gossypium hirsutum) plants. Bollworm larvae reared on T2 plants of the ds6-3 line exhibited drastically retarded growth, and the transgenic plants were less damaged by bollworms than the control. Quantitative reverse-transcription polymerase chain reaction (RT-PCR) showed that the CYP6AE14 expression level was reduced in the larvae as early as 4 h after feeding on the transgenic plants; accordingly, the CYP6AE14 protein level dropped. These results demonstrated that transgenic cotton plants expressing dsCYP6AE14 acquired enhanced resistance to cotton bollworms, and that RNAi technology can be used for engineering insect-proof cotton cultivar.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
RNA de Cadeia Dupla
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Controle Biológico de Vetores
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Plantas Geneticamente Modificadas
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Gossypium
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Sistema Enzimático do Citocromo P-450
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Lepidópteros
Limite:
Animals
Idioma:
En
Revista:
Transgenic Res
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2011
Tipo de documento:
Article