Highly consistent, fully representative mRNA-Seq libraries from ten nanograms of total RNA.
Biotechniques
; 49(6): 898-904, 2010 Dec.
Article
em En
| MEDLINE
| ID: mdl-21143212
Preparation of an Illumina sequencing library for gene expression analysis (mRNA-Seq) requires microgram amounts of starting total RNA or PCR-based amplification. Here we describe a protocol based on T7 linear RNA amplification that does not introduce significant bias, requires only 10 ng total RNA, and generates a directional, fully representative, whole-transcript mRNA-Seq Illumina library that is highly consistent across over three orders of magnitude of input RNA.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Biblioteca Gênica
/
Técnicas de Amplificação de Ácido Nucleico
Limite:
Humans
Idioma:
En
Revista:
Biotechniques
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Estados Unidos