Your browser doesn't support javascript.
loading
Inhibition of swine leukocyte antigen-I presentation in transgenic mini-pig cell lines by expressing human cytomegalovirus US6.
Yoo, J Y; Choi, K M; Hong, S P; Kim, S H; Park, K-W; Seol, J G.
Afiliação
  • Yoo JY; MGEN, Inc., Seoul, Korea.
Transplant Proc ; 42(10): 4648-50, 2010 Dec.
Article em En | MEDLINE | ID: mdl-21168753
ABSTRACT
Xenotransplantation using porcine organs may resolve the chronic shortage of donor organs for clinical transplantation if significant immunologic barriers can be overcome. A xenograft can be rejected by T cells, especially CD8(+) cytotoxic T lymphocytes (CTL)-mediated responses, as these cells show cytotoxicity against xenografts by recognition of swine leukocyte antigen (SLA)-I. Peptide translocation is inhibited by the endoplasmic reticulum-resident human cytomegalovirus (HCMV) glycoprotein unique short (US) 6, due to alterations of the transporter associated with antigen processing loading onto MHC class I for antigen presentation to CD8(+) CTL. In this study we transfected the US6 gene into minipig fetal fibroblasts establishing three US6 clonal cell lines. Flow cytometry analysis of US6 clonal cell lines demonstrated a substantial reduction in SLA-I expression. The level of SLA-I expression in US6 clones was decreased to 56.3% compared with the control 42.7%. In CTL assays, the rate of CD8(+) CTL-mediated cytotoxicity was significantly reduced to 35.2% ± 11.7% compared with the control, 79.9% ± 6.5%, (P < .01). These results suggested that HCMV viral protein US6 suppresses the presentation of SLA-I on pig fetal fibroblast cells. This strategy might be used in transgenic pig production to protect porcine organs from CTL-mediated immune rejection.
Assuntos

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Proteínas Virais / Antígenos de Histocompatibilidade Classe II / Proteínas de Ligação a RNA Limite: Animals / Humans Idioma: En Revista: Transplant Proc Ano de publicação: 2010 Tipo de documento: Article

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Proteínas Virais / Antígenos de Histocompatibilidade Classe II / Proteínas de Ligação a RNA Limite: Animals / Humans Idioma: En Revista: Transplant Proc Ano de publicação: 2010 Tipo de documento: Article