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Development of a cellular assay system to study the genome replication of high- and low-risk mucosal and cutaneous human papillomaviruses.
Geimanen, Jelizaveta; Isok-Paas, Helen; Pipitch, Regina; Salk, Kristiina; Laos, Triin; Orav, Marit; Reinson, Tormi; Ustav, Mart; Ustav, Mart; Ustav, Ene.
Afiliação
  • Geimanen J; Department of Biomedical Technology, Institute of Technology, University of Tartu, Nooruse 1, 50411, Tartu, Estonia.
J Virol ; 85(7): 3315-29, 2011 Apr.
Article em En | MEDLINE | ID: mdl-21248030
ABSTRACT
We found that recircularized high-risk (type 16 and 18) and low-risk mucosal (type 6b and 11) and cutaneous (type 5 and 8) human papillomavirus (HPV) genomes replicate readily when delivered into U2OS cells by electroporation. The replication efficiency is dependent on the amount of input HPV DNA and can be followed for more than 3 weeks in proliferating cell culture without selection. Cotransfection of recircularized HPV genomes with a linear G418 resistance marker plasmid has allowed subcloning of cell lines, which, in a majority of cases, carry multicopy episomal HPV DNA. Analysis of the HPV DNA status in these established cell lines showed that HPV genomes exist in these cells as stable extrachromosomal oligomers. When the cell lines were cultivated as confluent cultures, a 3- to 10-fold amplification of the HPV genomes per cell was induced. Two-dimensional (2D) agarose gel electrophoresis confirmed amplification of mono- and oligomeric HPV genomes in these confluent cell cultures. Amplification occurred as a result of the initiation of semiconservative two-dimensional replication from one active origin in the HPV oligomer. Our data suggest that the system described here might be a valuable, cost-effective, and efficient tool for use in HPV DNA replication studies, as well as for the design of cell-based assays to identify potential inhibitors of all stages of HPV genome replication.
Assuntos

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Papillomaviridae / Pele / Replicação Viral / Infecções por Papillomavirus / Mucosa Tipo de estudo: Etiology_studies / Prognostic_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: J Virol Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Estônia

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Papillomaviridae / Pele / Replicação Viral / Infecções por Papillomavirus / Mucosa Tipo de estudo: Etiology_studies / Prognostic_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: J Virol Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Estônia