Artificial duplicate reads in sequencing data of 454 Genome Sequencer FLX System.
Acta Biochim Biophys Sin (Shanghai)
; 43(6): 496-500, 2011 Jun.
Article
em En
| MEDLINE
| ID: mdl-21543404
ABSTRACT
The 454 Genome Sequencer (GS) FLX System is one of the next-generation sequencing systems featured by long reads, high accuracy, and ultra-high throughput. Based on the mechanism of emulsion PCR, a unique DNA template would only generate a unique sequence read after being amplified and sequenced on GS FLX. However, biased amplification of DNA templates might occur in the process of emulsion PCR, which results in production of artificial duplicate reads. Under the condition that each DNA template is unique to another, 3.49%-18.14% of total reads in GS FLX-sequencing data were found to be artificial duplicate reads. These duplicate reads may lead to misunderstanding of sequencing data and special attention should be paid to the potential biases they introduced to the data.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Análise de Sequência de DNA
/
Falha de Equipamento
Limite:
Humans
Idioma:
En
Revista:
Acta Biochim Biophys Sin (Shanghai)
Assunto da revista:
BIOFISICA
/
BIOQUIMICA
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
China