[Relationship between XRCC3 gene polymorphisms and lung cancer].

ABSTRACT

OBJECTIVE: To explore the relationship between XRCC3 and lung cancer after selecting tag SNPs from HapMap database and reconstructing haplotypes. METHODS: Using case-control study design, smoking condition were surveyed by questionare. Peripheral blood of cases and saliva or buccal cell for controls were collected, and then their DNA were extracted. XRCC3 tagSNPs were genotyped by PCR-RFLP. TagSNPs were selected by Haploview, HWE and LD were checked by LDA. Best genetic model and relationship between tagSNPs and lung cancer were analysed by SPSS, and haplotype frequencies and effects were computed by THESIAS. RESULTS: Carriers of rs861537 AG or AA genotype had a statistically significantly increased risk for lung cancer (OR = 1.48, 95% CI 1.17-1.86). Carriers of rs1799794 per G allele had a statistically significantly decreased risk for lung cancer (OR = 0.83, 95% CI 0.72-0.97); no significant association was observed between rs861539 and lung cancer. Smoke interacted with rs861537 polymorphism. Compared with CGG haplotype, carriers of CAA had a statistically significantly increased risk for lung cancer (OR = 1.32, 95% CI 1.11-1.57). CONCLUSION: XRCC3 gene polymorphisms were associated with lung cancer.